EP0153058A2 - Pipette mit einstellbarem Inhalt - Google Patents

Pipette mit einstellbarem Inhalt Download PDF

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Publication number
EP0153058A2
EP0153058A2 EP19850300680 EP85300680A EP0153058A2 EP 0153058 A2 EP0153058 A2 EP 0153058A2 EP 19850300680 EP19850300680 EP 19850300680 EP 85300680 A EP85300680 A EP 85300680A EP 0153058 A2 EP0153058 A2 EP 0153058A2
Authority
EP
European Patent Office
Prior art keywords
limiter
piston rod
pipette
grooves
piston
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP19850300680
Other languages
English (en)
French (fr)
Inventor
Mikael Fagerstedt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Thermo Fisher Scientific Oy
Original Assignee
Labsystems Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Labsystems Oy filed Critical Labsystems Oy
Publication of EP0153058A2 publication Critical patent/EP0153058A2/de
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops

Definitions

  • the present invention is concerned with a pipette provided with a piston, whose dosage volume is adjusted to the desired level by changing the stroke length of the piston.
  • the object of the present invention is to provide a continuously or almost continuously adjustable piston pipette whose volume adjustment is accurate but rapid.
  • the mechanism of adjustment of the pipette of the present invention has two parts: a rapid coarse adjustment and a precise fine adjustment.
  • the coarse adjustment acts upon the limiter of one extreme position of the piston and lengthens or shortens the stroke length of the piston stepwise.
  • the fine adjustment by means of the fine adjustment, the other limiter of the piston is shifted upwards or downwards.
  • the fine adjustment lengthens or shortens the stroke length by no more than the distance corresponding to the steps of the coarse adjustment.
  • the coarse adjustment is accomplished either so that the outer face of the piston rod is provided with grooves of different lengths and parallel to the rod, of which grooves any one can be positioned on a limiter pin provided on the pipette body and projecting inwards, whereby one end of the groove is closed and determines one of the extreme positions of the piston, or so that the corresponding grooves are provided on the body and the limiter pin on the piston rod.
  • the fine adjustment is accomplished so that the pipette body is provided with a second limiter which, together with a stop member provided at the piston rod, determines the other extreme position of the piston, the distance of the said limiter from the said stop member being adjustable.
  • a recommended fine adjustment mechanism is a limiter flange provided inside the body and determining the lower extreme position of the piston and, as its stop member, a sleeve fitted around the piston rod as moving by means of a threading.
  • the desired volume is first selected by placing the corresponding groove onto the limiter pin. Thereupon, the accurate volume is set by means of the fine adjustment member.
  • the coarse adjustment takes place rapidly, by means of one movement, and in this way the entire setting time becomes shorter than if the whole setting procedure were carried out by means of a fine adjustment member, whose operation is relatively slow.
  • the pipette comprises a body 1 and a cylinder part 2, which is threaded into the bottom portion of the body and to which a disposable container 3 is attached_by means of a friction joint.
  • a piston 4 and its seal ring 5 are fitted into the cylinder part 2.
  • the piston 4 relates to a sleeve 6 and to an extension 7 of the sleeve, the top end of the said extension 7 being provided with a press knob 8.
  • the outer face of the sleeve 6 is provided with grooves 10, 11, 12 and 13 parallel to the piston, having different lengths and extending to the top end 9 of the sleeve.
  • the extension 7 of the sleeve is provided with a fine adjustment threading 16 and with a threaded sleeve 17, which is fitted onto the said thread and whose bottom end widens as flange-shaped.
  • a ring 18 rests against the bottom flange of the threaded sleeve 17, which said ring 18 is pressed by a spiral spring 19, which is placed around the threaded sleeve and which is stronger than the spring 15, and the top end of the spring 19 is locked in its position by a sleeve 20.
  • a limiter flange 21 which determines the lower extreme position of the ring 18.
  • the coarse adjustment of the volume of the pipette is performed by, by means of the knob 8, pressing the piston 4 to its lower position and by, by means of the knob 8, turning the groove 10, 11, 12 or 13 corresponding to the desired volume onto the limiter pin 14.
  • the fine adjustment of the volume is carried out by turning by the sleeve 20, whereby the threaded sleeve 17 is also turned and the distance of the ring 18 from the limiter flange 21 becomes larger or smaller.
  • the sleeve 6 is preferably provided with ten grooves of different lengths, the lengths having equal steps from length to length, whereby an illustrative volume display can be easily constructed for the pipette.
  • a keyway 38 which rotates the ring 22 when the knob 8 is rotated.
  • numbers On the outer circumference of the ring 22, there are numbers, which can be read through the lower portion of the opening 23 provided in the body 1. This reading indicates the volume corresponding to the groove 10, 11, 12 or 13 that has been set.
  • the sleeve 20 is provided with a keyway 24, which rotates a number ring 25, whereby the numbers marked on the outer circumference of the said ring 25 can be read through the upper portion of the display opening 23. This reading indicates how much the volume indicated by the number ring 22 has been increased by means of fine adjustment.
  • notches 26 or 27 have been made into their end faces.
  • the notches 26 or 27 are jointly operative with springs 30 and 31 and balls 32 or 33 placed in recesses 28 or 29.
  • the movement of rotation of the number rings 22 or 25 is limited by means of pins 34 or 35 and grooves 36 or 37.
  • the grooves 36 or 37 do not extend around the entire circumference of the rings 22 or 25, and,consequently, the number rings can never revolve an entire full circle.
  • the piston 4 When the knob 8 is being depressed, the piston 4 is first pressed downwards when the spring 15 yields, until the ring 18 meets the limiter flange 21. This stroke length corresponds to the volume to be pipetted. In order to empty the pipette completely, the knob 8 is depressed further with a higher force, whereby the spring 19 yields until the threaded sleeve 17 meets the limiter pin 14.
EP19850300680 1984-02-14 1985-01-31 Pipette mit einstellbarem Inhalt Withdrawn EP0153058A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FI840579 1984-02-14
FI840579A FI840579A0 (fi) 1984-02-14 1984-02-14 Pipett med reglerbar volym

Publications (1)

Publication Number Publication Date
EP0153058A2 true EP0153058A2 (de) 1985-08-28

Family

ID=8518544

Family Applications (1)

Application Number Title Priority Date Filing Date
EP19850300680 Withdrawn EP0153058A2 (de) 1984-02-14 1985-01-31 Pipette mit einstellbarem Inhalt

Country Status (3)

Country Link
EP (1) EP0153058A2 (de)
JP (1) JPS60241937A (de)
FI (1) FI840579A0 (de)

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016976A1 (en) * 1990-05-04 1991-11-14 Biohit Oy Procedure for locking the adjustment means of a pipette, and pipette
US5454268A (en) * 1993-11-15 1995-10-03 Kim; Young S. Double-plunger liquid displacement syringe pipet
EP1197264A2 (de) * 2000-10-11 2002-04-17 Thermo Labsystems Oy Pipette à volume réglable
EP1514600A1 (de) * 2003-08-19 2005-03-16 Socorex Isba S.A. Mechanische Kolbenpipette
WO2005065360A2 (en) 2003-12-31 2005-07-21 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
EP2329885A1 (de) * 2009-12-07 2011-06-08 Fukae Kasei Co., Ltd. Pipettiervorrichtung
RU2530119C2 (ru) * 2010-05-28 2014-10-10 Эйкен Кагаку Кабусики Кайся Пипетка
CN110038659A (zh) * 2019-04-27 2019-07-23 李俊 对实验、检测所需液体进行精准定量抽取、移液的方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63149233U (de) * 1987-03-23 1988-09-30

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991016976A1 (en) * 1990-05-04 1991-11-14 Biohit Oy Procedure for locking the adjustment means of a pipette, and pipette
US5347878A (en) * 1990-05-04 1994-09-20 Biohit Oy Pipette with locking adjustment and method for using the same
US5454268A (en) * 1993-11-15 1995-10-03 Kim; Young S. Double-plunger liquid displacement syringe pipet
EP1197264A2 (de) * 2000-10-11 2002-04-17 Thermo Labsystems Oy Pipette à volume réglable
EP1197264A3 (de) * 2000-10-11 2002-11-27 Thermo Labsystems Oy Pipette à volume réglable
US7105130B2 (en) 2000-10-11 2006-09-12 Thermo Electron Oy Adjustable pipette
EP1514600A1 (de) * 2003-08-19 2005-03-16 Socorex Isba S.A. Mechanische Kolbenpipette
US7204163B2 (en) 2003-08-19 2007-04-17 Socorex Isba S.A. Mechanical piston pipette
EP1708814A2 (de) * 2003-12-31 2006-10-11 Rainin Instrument LLC. Volumeneinstellbare manuelle pipette mit schnell einstellbarem volumen
WO2005065360A2 (en) 2003-12-31 2005-07-21 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
EP1708814A4 (de) * 2003-12-31 2008-06-11 Rainin Instr Llc Volumeneinstellbare manuelle pipette mit schnell einstellbarem volumen
EP2329885A1 (de) * 2009-12-07 2011-06-08 Fukae Kasei Co., Ltd. Pipettiervorrichtung
US8646343B2 (en) 2009-12-07 2014-02-11 Fukae Kasei Co., Ltd. Pipette device
RU2530119C2 (ru) * 2010-05-28 2014-10-10 Эйкен Кагаку Кабусики Кайся Пипетка
CN110038659A (zh) * 2019-04-27 2019-07-23 李俊 对实验、检测所需液体进行精准定量抽取、移液的方法
CN110038659B (zh) * 2019-04-27 2021-04-27 日照奥基医学检验有限公司 对实验、检测所需液体进行精准定量抽取、移液的方法

Also Published As

Publication number Publication date
JPS60241937A (ja) 1985-11-30
FI840579A0 (fi) 1984-02-14

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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AK Designated contracting states

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STAA Information on the status of an ep patent application or granted ep patent

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18D Application deemed to be withdrawn

Effective date: 19870803

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Inventor name: FAGERSTEDT, MIKAEL